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VERSION:2.0
CALSCALE:GREGORIAN
PRODID:UW-Madison-Physics-Events
BEGIN:VEVENT
SEQUENCE:1
UID:UW-Physics-Event-4723
DTSTART:20180213T180500Z
DTEND:20180213T190000Z
DTSTAMP:20260419T055151Z
LAST-MODIFIED:20180116T220144Z
LOCATION:4274 Chamberlin (Refreshments will be served)
SUMMARY:Complexity in gene editing outcomes with defined CRISPR nanopa
 rticles\, Chaos & Complex Systems Seminar\, Kris Saha\, UW Department 
 of Biomedical Engineering
DESCRIPTION:Writing specific DNA sequences into the human genome is ch
 allenging with gene-editing reagents\, since most of the edited sequen
 ces contain various imprecise insertions or deletions of DNA sequence.
  Only a minor of sequences produced contain the desired sequence. We d
 eveloped a modular RNA aptamer-streptavidin strategy\, termed S1mplex\
 , to complex CRISPR-Cas9 ribonucleoproteins with a nucleic acid donor 
 template. In human cells\, tailored S1mplexes increase the ratio of pr
 ecisely edited to imprecisely edited alleles up to 18-fold higher than
  standard gene-editing methods\, and enrich cell populations containin
 g multiplexed precise edits up to 42-fold. Topics related to the compl
 exity seen in the sequence outcomes will be discussed. Advances in red
 ucing the complexity of sequence outcomes could greatly reduce the tim
 e and cost of in vitro or ex vivo gene-editing applications in precisi
 on medicine and drug discovery and aid in the development of increased
  and serial dosing regimens for somatic gene editing in vivo.
URL:https://www.physics.wisc.edu/events/?id=4723
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